Seminars & Lectures
| * TITLE | Statistical analysis of self-assembly of DNA tile lattice | ||||||
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| * DATE / TIME | 2015-03-05, 16:00pm | ||||||
| * ABSTRACT | |||||||
| We fabricate an two dimensional self-assembled DNA lattice and perform the statistical analysis for the DNA tile pattern. Double-crossover (DX) DNA tile is used as a elementary building unit. By imbedding hairpin structure two types of DX tile are prepared, the one has an additional hairpin structure and the other has not. The DX tile with hairpin structure has slightly higher folding temperature than the one without hairpin structure. The so-called sticky ends of five bases of oligonucleotides plays the role of tile-tile binding interaction. Several DX DNA tile lattice are made under the systematic change of experimental conditions: thermal annealing temperature and concentration of DNA strands. DNA tile structures are visualized by the Atomic Force Microscope (AFM) Imaging Method. In order to analyze the DNA tile pattern statistical mathematical model are applied and extract the binding strength between the two tiles: Each DX tile in the AFM images is identified and assigned to a binary number 1 or -1 depending on that the tile has hairpin or not, respectively. The relationship between the extracted parameters and experimental condition are discussed, looking forward to unveiling the principle of large-scale organization of DNA tile lattice. ∗To whom correspondence should be addressed †Department of Physics, Sungkyunkwan University, Suwon 440-746, Korea ‡Devision of Liberal Arts and Sciences, Gwangju Institute Science and Technology (GIST), Gwangju 500-712, Korea ¶SKKU Advanced Institute Nanotechnology (SAINT), Sunkyunkwan University, Suwon 440-746, korea 1 |
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